Atrenta, a San Jose start-up that offers chip design analysis software using standard SpyGlass technology, has raised $16 million in venture capital.

Investor Growth Capital led this fourth round of financing for Atrenta, while Samsung Ventures, Hercules Technology Growth Capital also became new investors. Existing investors Venrock Associates, TL Ventures, Smart Technology Ventures, Investcorp Technology Ventures and Finaventures participated.

In conjunction with the financing, Jose Suarez of Investor Growth Capital has joined Atrenta’s Board, and Bill Byun of Samsung Ventures joins as an observer on the Board.

The company says it has more than a hundred customers, including “all of the top semiconductor companies in the world.”

The company has already raised $43 million.